TUBB5/Beta V Tubulin Polyclonal Antibody

$Lane 1: Rat Cerebellum Lysate;Lane 2: Mouse Cerebellum Lysate;Lane 3: Mouse Cerebrum Lysate;Lane 4: Rat Cerebrum Lysate;Lane 5:Mouse Ovary Lysate;Lane 6:Mouse Heart Lysate;Lane 7: Rat Heart Lysate probed with TUBB5 Polyclonal Antibody, Unconjugated (bs-12863R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C\r\n\r\n$

Lane 1: Rat Cerebellum Lysate;Lane 2: Mouse Cerebellum Lysate;Lane 3: Mouse Cerebrum Lysate;Lane 4: Rat Cerebrum Lysate;Lane 5:Mouse Ovary Lysate;Lane 6:Mouse Heart Lysate;Lane 7: Rat Heart Lysate probed with TUBB5 Polyclonal Antibody, Unconjugated (bs-12863R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C\r\n\r\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat
Xenopus laevis

Predicted Reactivity

Sheep
Zebrafish

Overview
Catalog #	bs-12863R
Product Name	TUBB5/Beta V Tubulin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat, Xenopus laevis
Predicted Reactivity	Sheep, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TUBB5/Beta V Tubulin
Immunogen Range	301-444/444
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm
Synonyms	beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; TUBB1; TUBB5; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; TBB5_HUMAN.
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a nonexchangeable site on the alpha chain. The highly acidic C terminal region may bind cations such as calcium.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500