Sohlh1 Polyclonal Antibody

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with Sohlh1 Polyclonal Antibody, Unconjugated (bs-12278R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with Sohlh1 Polyclonal Antibody, Unconjugated (bs-12278R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Predicted Reactivity

Human
Mouse
Rat
Cow
Pig
Horse

Overview
Catalog #	bs-12278R
Product Name	Sohlh1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Predicted Reactivity	Human, Mouse, Rat, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Sohlh1
Immunogen Range	51-118/328
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	402381
Subcellular location	Cytoplasm, Nucleus
Synonyms	GM110; Helix Loop Helix Protein TOHLH1; Sohlh1; SOLH1_HUMAN; Spermatogenesis- and oogenesis-specic basic helix-loop-helix-containing protein 1; TOHLH1.
Background	SOHLH1 is a 328 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one bHLH domain through which it may function as a transcription factor during oogenesis and spermatogenesis. The gene encoding SOHLH1 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, and Familial dysautonomia, are both associated with chromosome 9. Notably, chromosome 9 encompasses the largest interferon family gene cluster.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500