CHRM4/mAChR M4 Polyclonal Antibody

$A431 (Positive) and Huvec (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4\/mAChR M4 Antibody (bs-11998R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A431 (Positive) and Huvec (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4\/mAChR M4 Antibody (bs-11998R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCHRM4\/mAChR M4 Polyclonal Antibody(bs-11998R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCHRM4\/mAChR M4 Polyclonal Antibody(bs-11998R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Lane 1: Human U-2 OS cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A549 cell lysates probed with CHRM4 Polyclonal Antibody, Unconjugated (bs-11998R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U-2 OS cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A549 cell lysates probed with CHRM4 Polyclonal Antibody, Unconjugated (bs-11998R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Rabbit

Overview
Catalog #	bs-11998R
Product Name	CHRM4/mAChR M4 Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHRM4/mAChR M4
Immunogen Range	1-31/479
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1132
Swiss Prot	P08173
Subcellular location	Cell membrane
Synonyms	HM4; M4R; Muscarinic acetylcholine receptor M4; CHRM4
Background	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500