TLR5 Polyclonal Antibody

$Lane 1: Mouse Liver; Lane 2: Mouse Ovary; 40ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Liver; Lane 2: Mouse Ovary; 40ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Raw264.7 Cells; Lane 2: Human Hela Cells; 30ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Raw264.7 Cells; Lane 2: Human Hela Cells; 30ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-1197R
Product Name	TLR5 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse TLR5
Immunogen Range	701-810/873
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	53791
Swiss Prot	Q9JLF7
Subcellular location	Cell membrane
Synonyms	Toll-like receptor 5; Tlr5
Background	Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400