Collagen 2 Polyclonal Antibody

Lane 1: U2os lysates; Lane 2: MDA-MB-231 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: HepG2 cell lysates; Lane 2: 293T cell lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n$

Lane 1: HepG2 cell lysates; Lane 2: 293T cell lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

Applications

WB
ELISA
IHC-P

Reactivity

Human
Rat
Rabbit

Predicted Reactivity

Mouse
Chicken

Overview
Catalog #	bs-11929R
Product Name	Collagen 2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Rat, Rabbit
Predicted Reactivity	Mouse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Collagen II
Immunogen Range	1401-1487/1487
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Secreted, Extracellular matrix
Synonyms	Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1II; type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1II chain precursor; Collagen type II alpha 1 primary osteoarthritis spondyloepiphyseal dysplasia congenital; MGC131516; SEDC.
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate with specificities to collagens. The development of type specific is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400

Applications

Reactivity

Predicted Reactivity

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