LBX1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LBX1 Polyclonal Antibody, Unconjugated (bs-11867R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LBX1 Polyclonal Antibody, Unconjugated (bs-11867R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Applications

WB
ELISA

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Horse
Chicken
Zebrafish

Overview
Catalog #	bs-11867R
Product Name	LBX1 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Horse, Chicken, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LBX1
Immunogen Range	166-250/281
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10660
Swiss Prot	P52954
Subcellular location	Nucleus
Synonyms	Homeobox; HPX 6; HPX6 antibodyLady bird like homeobox; Ladybird homeobox 1; Ladybird homeobox homolog 1; Ladybird homeobox protein homolog 1; LBX 1; LBX 1H; LBX1H; Transcription factor LBX1; Transcription factor similar to D. melanogaster homeodomain protein lady bird late; LBX1_HUMAN.
Background	The human homolog of the Drosophila lady bird late gene, LBX1, is specifically expressed in the developing central nervous system and musculoskeletal system. The LBX1 gene has a restrictive expression pattern in the dorsal portion of the mantle of the spinal cord and the hindbrain of the CNS. In the developing mouse and chicken musculoskeletal system, Lbx1 is expressed in migrating limb muscle precursor cells. In addition, Lbx1 may regulate migratory patterns of limb muscle cell precursors and may be essential to dorsal identification of forelimb muscles.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000