WRB Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-WRB Polyclonal Antibody, Unconjugated (bs-11774R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \\tWRB Polyclonal Antibody, Unconjugated (bs-11774R) at 1:600 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining$

Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \\tWRB Polyclonal Antibody, Unconjugated (bs-11774R) at 1:600 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Rabbit

Overview
Catalog #	bs-11774R
Product Name	WRB Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human WRB/Tryptophan rich protein
Immunogen Range	71-174/174
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7485
Swiss Prot	O00258
Subcellular location	Nucleus, Cell membrane
Synonyms	CHD5; Tail-anchored protein insertion receptor WRB; Congenital heart disease 5 protein; Tryptophan-rich basic protein; WRB
Background	Receptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500