NPC2/Niemann Pick C2 Polyclonal Antibody

Mouse Lung lysates probed with NPC2 Polyclonal Antibody, Unconjugated (bs-11736R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Recombinant human NPC2 protein, N-His(bs-42193P) probed with NPC2 Polyclonal Antibody, Unconjugated (bs-11736R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human NPC2 protein, N-His(bs-42193P) probed with NPC2 Polyclonal Antibody, Unconjugated (bs-11736R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Cow
Sheep

Overview
Catalog #	bs-11736R
Product Name	NPC2/Niemann Pick C2 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Cow, Sheep
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Niemann Pick C2
Immunogen Range	20-80/151
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm, Secreted
Synonyms	EDDM1; Epididymal protein 1; Epididymal secretory protein; Epididymal secretory protein E1; HE1; Human epididymis-specic protein 1; Niemann-Pick disease type C2; Niemann-Pick disease type C2 protein; NPC2; NPC2_HUMAN; Tissue specic secretory protein.
Background	NPC2 is a secreted protein mapping against gene 14q24.3 (1,2). NPC2 regulates the lipid composition of sperm membranes during maturation in the epididymis(1,2). Mutations in the NPC2 gene may cause Nieman-Pick type C2 disease and frontal lobe atrophy (1,2,3). Nieman-Pick type C2 is a fatal hereditary disease characterized by defective lysosome release of cholesterol (3). The disease is caused by HE1 deficiency, a lysosmal protein proven to be undetectable in fibroblasts from NPC2 patients (3). This differentiates NPC2 from NPC1, as NPC1 has HE1 protein present (3).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000