PET112L Polyclonal Antibody

$Mouse kidney lysates probed with PET112L Polyclonal Antibody, unconjugated (bs-11667R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Mouse kidney lysates probed with PET112L Polyclonal Antibody, unconjugated (bs-11667R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

$Mouse heart lysates probed with PET112L Polyclonal Antibody, unconjugated (bs-11667R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Mouse heart lysates probed with PET112L Polyclonal Antibody, unconjugated (bs-11667R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow

Overview
Catalog #	bs-11667R
Product Name	PET112L Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PET112L
Immunogen Range	101-200/557
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm
Synonyms	Cytochrome oxidase assembly factor PET112 homolog; GATB_HUMAN; GLU-ADT subunit B; HSPC199; mitochondrial; PET112; PET112 homolog yeast; PET112-like; Probable glutamyl-tRNAGln amidotransferase subunit B.
Background	PET112L is the human homolog of the S. cerevisiae COX assembly protein pet112, a protein that is believed to play an important role in the translation of mitochondrial genes. PET112L, also known as HSPC199 or Glu-ADT subunit B (glutamyl-tRNA(Gln) amidotransferase subunit B), is a 557 amino acid protein belonging to the gatB/gatE family of proteins (GatB subfamily) and is believed to play a role in energy metabolism. Localizing to mitochondria, PET112L is expressed in tissues such as heart and muscle, which exhibit high rates of oxidative phosphorylation. The gene encoding PET112L is overexpressed in recurrent ependymoma.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500