Progestin Receptor Beta/MPRB Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progestin Receptor Beta Polyclonal Antibody, Unconjugated (bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progestin Receptor Beta Polyclonal Antibody, Unconjugated (bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Progestin Receptor Beta\/MPRB Antibody(bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Progestin Receptor Beta\/MPRB Antibody(bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Progestin Receptor Beta\/MPRB Antibody (bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Progestin Receptor Beta\/MPRB Antibody (bs-11410R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

$Lane 1: Mouse Hippocampus lysates; Lane 2: Mouse Medulld lysates; Lane 3: Rat Hippocampus lysates; Lane 4: Rat Medulld lysates probed with Progestin Receptor Beta\/MPRB Polyclonal Antibody, Unconjugated (bs-11410R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Hippocampus lysates; Lane 2: Mouse Medulld lysates; Lane 3: Rat Hippocampus lysates; Lane 4: Rat Medulld lysates probed with Progestin Receptor Beta\/MPRB Polyclonal Antibody, Unconjugated (bs-11410R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse
Rat
Dog
Sheep

Predicted Reactivity

Human
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-11410R
Product Name	Progestin Receptor Beta/MPRB Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse, Rat, Dog, Sheep
Predicted Reactivity	Human, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Progestin Receptor Beta
Immunogen Range	251-300/354
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	85315
Subcellular location	Cell membrane
Synonyms	C6orf33; LMPB1; Lysosomal membrane protein in brain 1; Membrane progestin receptor beta; mPR beta; MPRB; PAQR8; Progestin and adipoQ receptor family member 8; Progestin and adipoQ receptor family member VIII; MPRB_HUMAN.
Background	The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel membrane progestin receptor (mPR). This pathway inhibits adenylyl cyclase and reduces intracellular cAMP, and also activates mitogen-activated protein kinase to effect signal transduction pathways. Five distinct groups, designated Alpha, Beta, Gamma, Delta, comprise the mPR gene family. mPR Alpha, also designated progestin and adipo Theta receptor family member VII (PAQR7), consists of an extracellular N-terminus, an intracellular C-terminus and seven transmembrane domains. mPR Alpha is expressed in ovary, testis, placenta, uterus and bladder. mPR Beta, or progestin and adipo Theta receptor family member VIII (PAQR8), consists of eight putative transmembrane regions and an intracellular N-terminus that contains a leucine-rich motif. mPR Beta is a 354 amino acid protein expressed in brain and spinal cord. Both mPR Alpha and mPR Beta may be G protein-coupled receptors and may be involved in oocyte maturation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500