Overview |
bs-1133R-Biotin |
SPARC Polyclonal Antibody, Biotin Conjugated |
WB, ELISA, IHC-P, IHC-F |
Human, Mouse, Rat |
Dog, Cow, Pig, Horse, Rabbit |
Specifications |
Biotin |
Rabbit |
KLH conjugated synthetic peptide derived from human SPARC |
101-200/303 |
Polyclonal |
IgG |
1ug/ul |
Purified by Protein A. |
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
Store at -20°C for 12 months. |
Target |
6678 |
P09486 |
Secreted, Cell membrane, Extracellular matrix |
ON; SPARC; Basement-membrane protein 4; BM-4; Osteonectin; Secreted protein acidic and rich in cysteine |
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
Application Dilution |
WB |
1:300-5000 |
ELISA |
1:500-1000 |
IHC-P |
1:200-400 |
IHC-F |
1:100-500 |