HB9/HLXB9 Polyclonal Antibody

$RSC96 cells probed with HB9\/HLXB9 Polyclonal Antibody, Unconjugated (bs-11320R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

RSC96 cells probed with HB9\/HLXB9 Polyclonal Antibody, Unconjugated (bs-11320R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Lane 1: Mouse Pancreas tissue lysates; Lane 2: Rat Pancreas tissue lysates probed with HB9\/HLXB9 Polyclonal Antibody, Unconjugated (bs-11320R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Pancreas tissue lysates; Lane 2: Rat Pancreas tissue lysates probed with HB9\/HLXB9 Polyclonal Antibody, Unconjugated (bs-11320R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HB9\/HLXB9 Polyclonal Antibody(bs-11320R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HB9\/HLXB9 Polyclonal Antibody(bs-11320R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-11320R
Product Name	HB9/HLXB9 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HLXB9
Immunogen Range	231-330/401
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3110
Swiss Prot	P50219
Subcellular location	Nucleus
Synonyms	HB9; HLXB9; SCRA1; HOXHB9; Motor neuron and pancreas homeobox protein 1; Homeobox protein HB9; MNX1
Background	Putative transcription factor involved in pancreas development and function.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500