5-HTR1A Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-5-HTR1A\/HTR1A Polyclonal Antibody, Unconjugated (bs-1124R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-5-HTR1A\/HTR1A Polyclonal Antibody, Unconjugated (bs-1124R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysate; Lane 3: Mouse Lymph Lysates; Lane 4: Rat Brain Lysates; Lane 5: Rat Cerebellum Lysates. Probed with 5HT1A Receptor polyclonal Antibody, unconjugated (bs-1124R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysate; Lane 3: Mouse Lymph Lysates; Lane 4: Rat Brain Lysates; Lane 5: Rat Cerebellum Lysates. Probed with 5HT1A Receptor polyclonal Antibody, unconjugated (bs-1124R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Horse
Rabbit

Overview
Catalog #	bs-1124R
Product Name	5-HTR1A Polyclonal Antibody
Applications	WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat 5-HTR1A
Immunogen Range	301-400/422
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	24473
Swiss Prot	P19327
Subcellular location	Cell membrane
Synonyms	5HT1A; RAT5HT1A; 5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; Serotonin receptor 1A; Htr1a
Background	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200