CACNA1F Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CACNA1F Polyclonal Antibody, Unconjugated (bs-11245R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CACNA1F Polyclonal Antibody, Unconjugated (bs-11245R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

$Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CACNA1F Polyclonal Antibody, Unconjugated (bs-11245R) at 1:400 overnight at 4\u00b0C, followed by a biotin conjugated secondary for 20 minutes and Cy3 streptavidin at 1:500 for 40 minutes and DAPI staining$

Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CACNA1F Polyclonal Antibody, Unconjugated (bs-11245R) at 1:400 overnight at 4\u00b0C, followed by a biotin conjugated secondary for 20 minutes and Cy3 streptavidin at 1:500 for 40 minutes and DAPI staining

Applications

ELISA
IHC-P
IF(IHC-P)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Sheep
Pig

Overview
Catalog #	bs-11245R
Product Name	CACNA1F Polyclonal Antibody
Applications	ELISA, IHC-P, IF(IHC-P)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CACNA1F
Immunogen Range	1001-1100/1977
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	778
Swiss Prot	O60840
Subcellular location	Cell membrane
Synonyms	CACNA 1F; CACNAF; CACNAF1; Calcium channel voltage dependent alpha 1F subunit; Calcium channel voltage dependent L type alpha 1F subunit; Cav1.4; Cav1.4alpha1; COD 3; COD3; CORDX 3; CORDX; CORDX3; CSNB2A; CSNBX 2; CSNBX2; JM 8; JM8; JMC 8; JMC8; Voltage ated calcium channel subunit alpha Cav1.4; Voltage ependent L ype calcium channel subunit alpha F; CAC1F_HUMAN.
Background	Voltage-dependent Ca2+ channels mediate Ca2+ entry into excitable cells in response to membrane depolarization, and they are involved in a variety of Ca2+-dependent processes, including muscle contraction, hormone or neurotransmitter release and gene expression.Ca2+ currents are characterized on the basis of their biophysical and pharmacologic properties and include L-, N-, T-, P-, Q-, and R- types. L-type Ca2+ currents initiate muscle contraction, endocrine secretion, and gene transcription, and can be regulated through second-messenger activated protein phosphorylation pathways. L-type calcium channels may form macromolecular signaling complexes with G protein-coupled receptors, thereby enhancing the selectivity of regulating specific targets.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200