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IQGAP1 Polyclonal Antibody

Formalin-fixed and rat lung tissue labeled with Anti-IQGAP1 Polyclonal Antibody, Unconjugated (bs-1109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and rat lung tissue labeled with Anti-IQGAP1 Polyclonal Antibody, Unconjugated (bs-1109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and rabbit placenta tissue labeled with Anti-IQGAP1 Polyclonal Antibody, Unconjugated (bs-1109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and rabbit placenta tissue labeled with Anti-IQGAP1 Polyclonal Antibody, Unconjugated (bs-1109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U87MG cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IQGAP1 Polyclonal Antibody(bs-1109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U87MG cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IQGAP1 Polyclonal Antibody(bs-1109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat
  • Rabbit

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-1109R
Product Name IQGAP1 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Rat, Rabbit
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IQGAP1
Immunogen Range 1451-1550/1657
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8826
Swiss Prot P46940
Subcellular location Nucleus, Cell membrane
Synonyms SAR1; p195; HUMORFA1; Ras GTPase-activating-like protein IQGAP1; IQGAP1; KIAA51
Background Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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