MRP2 Polyclonal Antibody

Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin-embedded human liver carcinoma labeled with Anti-MRP2 Polyclonal Antibody, Unconjugated(bs-1092R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded human liver carcinoma labeled with Anti-MRP2 Polyclonal Antibody, Unconjugated(bs-1092R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$HepG2 cell lysates probed with ABCC2\/MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.$

HepG2 cell lysates probed with ABCC2\/MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Mouse liver lysates probed with ABCC2\/MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.$

Mouse liver lysates probed with ABCC2\/MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Panc-1 cell lysates probed with MRP2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MRP2) Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MRP2) Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1092R
Product Name	MRP2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MRP2
Immunogen Range	485-615/1545
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1244
Swiss Prot	Q92887
Subcellular location	Cell membrane
Synonyms	DJS; MRP2; cMRP; ABC3; CMOAT; Canalicular multispecific organic anion transporter 1; ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2; ABCC2; CMOAT1
Background	Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200