Cytokeratin 5 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat tongue labeled with Rabbit Anti Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat tongue labeled with Rabbit Anti Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$L1 rat heart lysates L2 rat heart lysates probed with Anti Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 64kD. Observed band size:64kD.\\n$

L1 rat heart lysates L2 rat heart lysates probed with Anti Cytokeratin 5\/CK5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 64kD. Observed band size:64kD.\\n

Lane 1: Mouse Skin lysates; Lane 2: Mouse Uterus lysates; Lane 3: Mouse Urinary bladder lysates; Lane 4: Mouse Esophagus lysates; Lane 5: Rat Skin lysates; Lane 6: Rat Uterus lysates; Lane 7: Rat Urinary bladder lysates; Lane 8: Rat Esophagus probed with Cytokeratin 5 Polyclonal Antibody, Unconjugated (bs-1060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-1060R
Product Name	Cytokeratin 5 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CK5
Immunogen Range	331-430/590
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3852
Subcellular location	Cytoplasm, Nucleus, Extracellular matrix
Synonyms	Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; CK 5; CK5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; DDD; EBS 2; EBS2; K5; Keratin 5 epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types; Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5; Cytokeratin-5; Cytokeratin5; DDD; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; Keratin 5; Keratin; Keratin Type II Cytoskeletal 5; Keratin5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5.
Background	Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200