GDNF Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 23kD. Observed band size: 23kD.

Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GDNF Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Rabbit

Predicted Reactivity

Dog
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-1024R
Product Name	GDNF Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine, Rabbit
Predicted Reactivity	Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GDNF
Immunogen Range	121-211/211
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2668
Swiss Prot	P39905
Subcellular location	Secreted
Synonyms	ATF1; ATF2; HSCR3; HFB1-GDNF; Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF; GDNF
Background	Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200