CD5 Polyclonal Antibody, PE Conjugated

$Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNFRSF5 Polyclonal Antibody, FITC Conjugated\uff08bs-10218R-PE\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNFRSF5 Polyclonal Antibody, FITC Conjugated\uff08bs-10218R-PE\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

$MCF7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD5 Polyclonal Antibody(bs-10218R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

MCF7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD5 Polyclonal Antibody(bs-10218R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Horse
Rabbit

Overview
Catalog #	bs-10218r-pe-100ul
Product Name	CD5 Polyclonal Antibody, PE Conjugated
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Horse, Rabbit
Specifications
Conjugation	PE
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD5
Immunogen Range	221-320/495
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	921
Swiss Prot	P06127
Subcellular location	Cell membrane
Synonyms	T1; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5
Background	May act as a receptor in regulating T-cell proliferation.
Application Dilution
WB	1:300-5000
FCM	1:20-100

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