Desmoglein 2 Polyclonal Antibody

Formalin-fixed and paraffin embedded labeled with Anti-Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Desmoglein 2 Polyclonal Antibody, Unconjugated (bs-10152R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-10152R
Product Name	Desmoglein 2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Desmoglein 2
Immunogen Range	51-150/1118
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1829
Swiss Prot	Q14126
Subcellular location	Cell membrane
Synonyms	HDGC; CDHF5; ARVC10; ARVD10; CMD1BB; Desmoglein-2; Cadherin family member 5; DSG2
Background	Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500