CXCR4 Polyclonal Antibody

$L1 rat brain lysates L2 rat kidney lysates probed with Anti- CD184\/CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 39kD.Observed band 39kD$

L1 rat brain lysates L2 rat kidney lysates probed with Anti- CD184\/CXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 39kD.Observed band 39kD

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184\/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184\/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184\/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184\/CXCR4 Polyclonal Antibody (bs-1011R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse lung lysates probed with Anti-CXCR4 Polyclonal Antibody (bs-1011R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Human U937 cells probed withCXCR4 Polyclonal Antibody, Unconjugated (bs-1011R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Rabbit

Predicted Reactivity

Overview
Catalog #	bs-1011R
Product Name	CXCR4 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Rabbit
Predicted Reactivity	Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CXCR4
Immunogen Range	201-294/352
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7852
Subcellular location	Cell membrane
Synonyms	C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; Lipopolysaccharide-associated protein 3; LAP-3; LPS-associated protein 3; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD184; CXCR4
Background	Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200