DDX58 Polyclonal Antibody

$Lane 1: Raw264.7 Cells; Probed with DDX58 Polyclonal Antibody, Unconjugated (bs-0993R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C. The lower band is assumed to be smaller isoform.$

Lane 1: Raw264.7 Cells; Probed with DDX58 Polyclonal Antibody, Unconjugated (bs-0993R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C. The lower band is assumed to be smaller isoform.

Applications

WB
ELISA
IHC-P

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig

Overview
Catalog #	bs-0993R
Product Name	DDX58 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DDX58
Immunogen Range	201-300/925
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23586
Swiss Prot	O95786
Subcellular location	Cytoplasm
Synonyms	RIGI; RIG-I; RLR-1; SGMRT2; Probable ATP-dependent RNA helicase DDX58; DEAD box protein 58; RIG-I-like receptor 1; Retinoic acid-inducible gene 1 protein; RIG-1; Retinoic acid-inducible gene I protein; DDX58
Background	Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400