VE Cadherin Polyclonal Antibody

$Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD144\/VECD\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD144\/VECD\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(bs-0878R) 1:100, overnight at 4\u00b0C, The secondary antibody was diluted to 1:100 for 120 minutes at 37\u00b0C.$

Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(bs-0878R) 1:100, overnight at 4\u00b0C, The secondary antibody was diluted to 1:100 for 120 minutes at 37\u00b0C.

A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.

Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

$Mouse heart lysates probed with VE Cadherin Polyclonal Antibody, unconjugated (bs-0878R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Mouse heart lysates probed with VE Cadherin Polyclonal Antibody, unconjugated (bs-0878R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Mouse kidney lysates probed with VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 3ug for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0878R
Product Name	VE Cadherin Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse vascular endothelial cadherin
Immunogen Range	601-700/784
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12562
Swiss Prot	P55284
Subcellular location	Cell membrane
Synonyms	7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA48225; Cadherin-5; Vascular endothelial cadherin; VE-cadherin; Cdh5
Background	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500