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AKT1/2/3 (Ser472/Ser473/Ser474) Antibody, AbBy Fluor® 647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Chicken
  • Rabbit
  • Fish
  • Others

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
Overview
Catalog # bs-0876R-BF647
Product Name AKT1/2/3 (Ser472/Ser473/Ser474) Antibody, AbBy Fluor® 647 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others
Predicted Reactivity Dog, Cow, Sheep
Specifications
Conjugation AbBy Fluor® 647
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Modification Site Ser473
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24185
Swiss Prot P47196
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Background AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200