Fibulin 5 Polyclonal Antibody

L1 rat brain, L2 human colon carcinoma lysates probed (bs-0810R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 50kDa.

$Paraformaldehyde-fixed, paraffin embedded Rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fibulin 5 Polyclonal Antibody, Unconjugated (bs-0810R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fibulin 5 Polyclonal Antibody, Unconjugated (bs-0810R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Lane 1: Mouse kidney lysates; Lane 2: Mouse large intestine lysates; probed with Fibulin 5 Polyclonal Antibody, unconjugated (bs-0810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse kidney lysates; Lane 2: Mouse large intestine lysates; probed with Fibulin 5 Polyclonal Antibody, unconjugated (bs-0810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Lane 1: Lovo cell lysates probed with Fibulin 5 Polyclonal Antibody, Unconjugated (bs-0810R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-0810R
Product Name	Fibulin 5 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Fibulin 5
Immunogen Range	101-200/448
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10516
Swiss Prot	Q9UBX5
Subcellular location	Secreted
Synonyms	EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5; Fibulin-5; Developmental arteries and neural crest EGF-like protein; Urine p50 protein; FBLN5; UNQ184/PRO210
Background	Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400