Smad2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse kidney lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Mouse placenta lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Placenta lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Heart lysates; Lane 8: Rat Testis lysates; Lane 9: Rat Kidney lysates; Lane 10: Human Hela cell lysates; Lane 11: Human HT1080 cell lysates; Lane 12: Human Jurkat cell lysates; Lane 13: Mouse RAW264.7 cell lysates; Lane 14: Human HL-60 cell lysates probed with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat
Rabbit
Goat
Others

Predicted Reactivity

Dog
Cow
Chicken

Overview
Catalog #	bs-0718R
Product Name	Smad2 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat, Rabbit, Goat, Others
Predicted Reactivity	Dog, Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Smad2
Immunogen Range	21-120/467
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4087
Swiss Prot	Q15796
Subcellular location	Cytoplasm, Nucleus
Synonyms	JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2; SMAD family member 2; SMAD 2; Smad2
Background	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500