Connexin 43 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

$MCF-7 cells were stained with Connexin 43 Polyclonal Antibody, Unconjugated(bs-0651R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.$

MCF-7 cells were stained with Connexin 43 Polyclonal Antibody, Unconjugated(bs-0651R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Hela cells probed with Connexin 43 Polyclonal Antibody, unconjugated (bs-0651R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Mouse Bladder lysates probed with Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Tissue\/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Connexin 43) polyclonal Antibody, Unconjugated (bs-0651R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Connexin 43) polyclonal Antibody, Unconjugated (bs-0651R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Dog

Predicted Reactivity

Cow
Chicken

Overview
Catalog #	bs-0651R
Product Name	Connexin 43 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Dog
Predicted Reactivity	Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Connexin-43
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2697
Swiss Prot	P17302
Subcellular location	Cytoplasm, Cell membrane, Extracellular matrix
Synonyms	HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1; Gap junction alpha-1 protein; Connexin-43; Gap junction 43 kDa heart protein; GJA1
Background	Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200