CD8 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated(bs-0648R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.

Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody (bs-0648R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

$Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Spleen; 40ug loaded in each lane; Probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Spleen; 40ug loaded in each lane; Probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Lane 1: Jurkat cell lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: mCD8a overexpression 293T cell lysates probed with CD8a Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0648R
Product Name	CD8 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CD8
Immunogen Range	51-150/247
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12525
Swiss Prot	P01731
Subcellular location	Cell membrane
Synonyms	Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Lyt2
Background	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200