CD4 Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n$

Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n

Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.

Image provided by the Independent Validation Program, badge number 029634:Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 followed by conjugation to the secondary antibody and DAB staining

Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with (bs-0647R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

$Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Lane 1: HL60 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HL60 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: U937 Cells; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Spleen; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Spleen; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Lymph Node; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Lymph Node; Probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Human HL60 Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 1 X PBS \/ 2% BSA \/ 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with CD4 Polyclonal Antibody Unconjugated (bs-0647R; green) at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-FITC (bs-0295G-FITC), was used for 40 min at room temperature. Primary antibody staining is compared to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).$

Human HL60 Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 1 X PBS \/ 2% BSA \/ 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with CD4 Polyclonal Antibody Unconjugated (bs-0647R; green) at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-FITC (bs-0295G-FITC), was used for 40 min at room temperature. Primary antibody staining is compared to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).

Lane 1: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human U937 cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Pig
Chicken

Predicted Reactivity

Dog
Cow
Sheep
Guinea Pig

Overview
Catalog #	bs-0647R
Product Name	CD4 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Pig, Chicken
Predicted Reactivity	Dog, Cow, Sheep, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)
Immunogen Range	385-457/457
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	24932
Swiss Prot	P01730
Subcellular location	Cell membrane
Synonyms	T-cell surface glycoprotein CD4; Cd4; T-cell surface antigen T4/Leu-3; W3/25 antigen
Background	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200