Estrogen Sulfotranferase Antibody

$Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$HepG2 probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

HepG2 probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

$MCF-7 Cell lysates probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

MCF-7 Cell lysates probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Applications

WB
ELISA
IHC-P

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Pig
Horse
Rabbit

Overview
Catalog #	bs-0629R
Product Name	Estrogen Sulfotranferase Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Estrogen Sulfotranferase
Immunogen Range	51-145/294
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6783
Swiss Prot	P49888
Subcellular location	Cytoplasm
Synonyms	EST; STE; EST-1; ST1E1; Estrogen sulfotransferase; Sulfotransferase 1E1; Sulfotransferase, estrogen-preferring; SULT1E1
Background	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 2 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400