VCAM-1 Polyclonal Antibody

Lane 1: Human VCAM1 protein; Probed with Rabbit Anti-VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.

$Mouse spleen cells (dark blue) were fixed with 2% paraformaldehyde for 10 min at 37\u2103. Primary antibody, Rabbit Anti-VCAM-1 antibody (bs-0396R, Green) at 1\u03bcg \/8x10^5 cells was incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then Goat Anti-rabbit IgG\/FITC (light blue) antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1\/200 dilution for 40 min at room temperature. Isotype control Rabbit IgG (orange) was analyzed under the same conditions.$

Mouse spleen cells (dark blue) were fixed with 2% paraformaldehyde for 10 min at 37\u2103. Primary antibody, Rabbit Anti-VCAM-1 antibody (bs-0396R, Green) at 1\u03bcg \/8x10^5 cells was incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then Goat Anti-rabbit IgG\/FITC (light blue) antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1\/200 dilution for 40 min at room temperature. Isotype control Rabbit IgG (orange) was analyzed under the same conditions.

Lane 1: Human Siha cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with VCAM-1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0396R
Product Name	VCAM-1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse VCAM-1
Immunogen Range	640-739/739
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22329
Swiss Prot	P29533
Subcellular location	Cell membrane
Synonyms	CD106; Vcam-1; Vascular cell adhesion protein 1; V-CAM 1; Vcam1
Background	Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200