GLUT4 Polyclonal Antibody

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-GLUT4 Polyclonal Antibody (bs-0384R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse heart lysates probed with Anti-GLUT4 Polyclonal Antibody (bs-0384R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

$Tissue\/cell: NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell: NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Heart lysates; Lane 3: Rat Heart lysates; Lane 4: Mouse Muscle lysates; Lane 5: Rat Muscle lysates probed with GLUT4 Polyclonal Antibody, Unconjugated (bs-0384R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Heart lysates; Lane 3: Rat Heart lysates; Lane 4: Mouse Muscle lysates; Lane 5: Rat Muscle lysates probed with GLUT4 Polyclonal Antibody, Unconjugated (bs-0384R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Pig
Sheep
Goat

Predicted Reactivity

Dog
Cow
Rabbit

Overview
Catalog #	bs-0384R
Product Name	GLUT4 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Pig, Sheep, Goat
Predicted Reactivity	Dog, Cow, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GLUT4
Immunogen Range	401-509/509
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6517
Swiss Prot	P14672
Subcellular location	Cytoplasm, Cell membrane
Synonyms	GLUT4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive; GLUT-4; SLC2A4
Background	GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200