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eNOS Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
  • Others

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Guinea Pig
Overview
Catalog # bs-0163R-Biotin
Product Name eNOS Polyclonal Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Human, Mouse, Rat, Others
Predicted Reactivity Dog, Cow, Sheep, Pig, Guinea Pig
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NOS-3
Immunogen Range 1105-1202/1203
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 4846
Swiss Prot P29474
Subcellular location Cytoplasm, Cell membrane
Synonyms eNOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3
Background Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500