eNOS Polyclonal Antibody

$Cultured endothelial cells labeled with Anti-Nos-3\/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.$

Cultured endothelial cells labeled with Anti-Nos-3\/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Others

Predicted Reactivity

Dog
Cow
Sheep
Pig
Guinea Pig

Overview
Catalog #	bs-0163R
Product Name	eNOS Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Others
Predicted Reactivity	Dog, Cow, Sheep, Pig, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NOS-3
Immunogen Range	1105-1202/1203
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4846
Swiss Prot	P29474
Subcellular location	Cytoplasm, Cell membrane
Synonyms	eNOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3
Background	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200