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HPV16 E6 protein (2E3) Monoclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)

Reactivity

  • Virus
Overview
Catalog # bsm-0990M-PerCP
Product Name HPV16 E6 protein (2E3) Monoclonal Antibody, PerCP Conjugated
Applications IF(IHC-P)
Reactivity Virus
Specifications
Conjugation PerCP
Host Mouse
Source KLH conjugated synthetic peptide derived from HPV16 E6 protein
Immunogen Range 85-158/158
Clonality Monoclonal
Clone # 2E3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1489078
Swiss Prot P03126
Synonyms Protein E6; E6
Background Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.
Application Dilution
IF(IHC-P) 1:50-200