Mycobacterium tuberculosis Ag85A/B Antibody

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Bacteria

Overview
Catalog #	bs-6460R
Product Name	Mycobacterium tuberculosis Ag85A/B Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody will detect both Mycobacterium tuberculosis Ag85A and Mycobacterium tuberculosis Ag85B
Reactivity	Bacteria
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Antigen 85 complex B
Immunogen Range	115-154/325
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	885785, 886132
Swiss Prot	P9WQP1, P9WQP3
Subcellular location	Secreted
Synonyms	M.tuberculosis;Antigen 85 complex B;30 kDa extracellular protein;Mycobacterium;Mycolyl transferase 85B; A85B_MYCTU;Antigen 85-B; 30 kDa extracellular protein; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B.
Background	The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200