NDV HN protein Polyclonal Antibody

$Antigen: bs-4529P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.$

Antigen: bs-4529P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.

Applications

WB
IHC-P

Reactivity

Virus
Others

Overview
Catalog #	bs-4529R
Product Name	NDV HN protein Polyclonal Antibody
Applications	WB, IHC-P
Reactivity	Virus, Others
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	recombinant protein corresponding to amino acids 49 to 577 out of the full length (577 amino acids) Newcastle disease virus Hemagglutinin-neuraminidase protein
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P32884
Subcellular location	Cell membrane
Synonyms	hemagglutinin-neuraminidase protein; newcastle disease virusNDV; HN protein; hemagglutinin-neuraminidase protein [Newcastle disease virus]; HN_NDVB; Newcastle disease virus HN protein; Newcastle disease virus hemagglutinin neuraminidase protein.
Background	The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in the process of infection. It attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion.
Application Dilution
WB	1:500-1000
IHC-P	1:200-400