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CD80 Polyclonal Antibody, APC-Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-2211R-APC-Cy5.5
Product Name CD80 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat CD80
Immunogen Range 251-321/321
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 25408
Swiss Prot G3V671
Subcellular location Cell membrane
Synonyms B7-1; Activation B7 1 antigen; Activation B71 antigen; B lymphocyte activation antigen B7; B7; BB1; CD 80; CD28 antigen ligand 1 B7 1 antigen; CD28 antigen ligand 1 B71 antigen; CD28LG; CD28LG1; CD80 antigen; CD80 antigen precursor;CD80 molecule; Costimulatory factor CD80; CTLA 4 counter receptor B7.1; CTLA-4 counter-receptor B7.1; LAB 7; LAB7; T lymphocyte activation antigen CD80; CD80_MOUSE.
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200