GABA Polyclonal Antibody

Formalin-fixed and rat brain tissue labeled with Anti-GABA Polyclonal Antibody, Unconjugated (bs-2252R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GABA Polyclonal Antibody, Unconjugated (bs-2252R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GABA Polyclonal Antibody, Unconjugated (bs-2252R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Rat
Others

Overview
Catalog #	bs-2252R
Product Name	GABA Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Rat, Others
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated GABA
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm, Secreted, Cell membrane
Synonyms	gamma-aminobutyric acid; gamma aminobutyric acid; 4 aminobutanoic acid; GABA; Gamma amino butyric acid.
Background	Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200