ELAVL1 (7A1) Monoclonal Antibody

Flow cytometric analysis of Jurkat cells with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Rat Spleen; Lane 2: Rat Heart; Lane 3: PC-12 Cells; Lane 4: Mouse Heart; Lane 5: Hela Cells; Probed with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Spleen; Lane 2: Rat Heart; Lane 3: PC-12 Cells; Lane 4: Mouse Heart; Lane 5: Hela Cells; Probed with ELAVL1 (7A1) Monoclonal Antibody (bsm-54135R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54135R
Product Name	ELAVL1 (7A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human HuR/ ELAVL1 aa 1-150
Clonality	Monoclonal
Clone #	7A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	1994
Swiss Prot	Q15717
Subcellular location	Cytoplasm, Nucleus
Synonyms	ELAV-like protein 1; Hu-antigen R; HuR; ELAVL1;
Background	RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400