| Overview |
| bsm-70425M |
| Connexin-43 (C-terminal region) Antibody |
| WB, ICC |
| The antibody detects a 43 kDa* protein corresponding to the apparent molecular mass of connexin-43 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells. |
| Human, Mouse, Rat, Chicken |
| Specifications |
| Unconjugated |
| Mouse |
| Clone M496 was generated from a peptide fragment in the C-terminal region of human connexin-43. This sequence is highly conserved in mouse and rat connexin-43. |
| Monoclonal |
| M496 |
| IgG1 |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| P17302 |
| Gap junction alpha-1 43 GJA1 CXA1 |
| Connexin-43 (Cx43, CXA1, Gap Junction α1) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels that cluster together to form gap junctions. Gap junction communication is critical for cell to cell communication during development and regulation of cell growth. Phosphorylation of connexin-43 is important in regulating both the assembly and the function of gap junctions. PKC phosphorylates Ser-368 in connexin-43 after activation of cells with phorbol esters. This phosphorylation event decreases cell-to-cell communication. In addition, the tyrosine kinase, c-Src, can interact with and phosphorylate Tyr-265 in connexin-43 leading to inhibition of gap junction communication. Thus, connexin-43 phosphorylation may be an important mode for regulating gap junctional communication. |
| Application Dilution |
| WB |
1:300-5000 |
| ICC |
1:100-500 |
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