Microtubule Associated Protein 2 (MAP2) Antibody

Applications

Reactivity

Human
Mouse
Rat
Bovine

Overview
Catalog #	bsm-70140M
Product Name	Microtubule Associated Protein 2 (MAP2) Antibody
Applications	WB, IHC, ICC
Specificity	Specific for endogenous levels of the ~280 kDa MAP2 protein.
Reactivity	Human, Mouse, Rat, Bovine
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified bovine MAP2.
Clonality	Monoclonal
Clone #	4H5
Isotype	IgG1
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	PBS + 50% glycerol and 5 mM NaN3
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	4133
Swiss Prot	P11137
Synonyms	DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody
Background	Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ? (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).
Application Dilution
WB	1:300-5000
IHC
ICC	1:100-500