Firefly Luciferase Recombinant Monoclonal Antibody

$Western blot analysis of (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH\/3T3 cell lysate; (4) C6 cell lysate. Using Firefly Luciferase (bsm-63023R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH\/3T3 cell lysate; (4) C6 cell lysate. Using Firefly Luciferase (bsm-63023R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IF(ICC)

Overview
Catalog #	bsm-63023r-100ul
Product Name	Firefly Luciferase Recombinant Monoclonal Antibody
Applications	WB, IF(ICC)
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Firefly luciferase
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P08659
Subcellular location	Cytoplasm
Synonyms	Luciferase; Firefly; Luciferin 4 monooxygenase.
Background	Produces green light with a wavelength of 562 nm.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200