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TRIM28 Recombinant Monoclonal Antibody, PerCP-Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63000r-percp-cy7-100ul
Product Name TRIM28 Recombinant Monoclonal Antibody, PerCP-Cy7 Conjugated
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TIF1 beta
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10155
Swiss Prot Q13263
Subcellular location Nucleus
Synonyms E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; KAP1; TF1B; RNF96; TIF1B; TRIM28.
Background Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200