PKC epsilon Recombinant Monoclonal Antibody

$Western blot analysis of Jurkat cell lysate. Using PKC epsilon (bsm-62955R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Jurkat cell lysate. Using PKC epsilon (bsm-62955R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62955r-100ul
Product Name	PKC epsilon Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PKC epsilon
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5581
Swiss Prot	Q02156
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	nPKC epsilon; PKCE; Pkcea; PRKCE; Protein kinase C epsilon; Protein kinase C epsilon type.
Background	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF