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Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62940r-cy3-100ul
Product Name Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, Cy3 Conjugated
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S5
Modification Site Ser5
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5430
Swiss Prot P24928
Subcellular location Nucleus
Synonyms POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS.
Background During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200