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Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, AbBy Fluor® 680 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62940r-bf680-100ul
Product Name Phospho-POLR2A (Ser5) Recombinant Monoclonal Antibody, AbBy Fluor® 680 Conjugated
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor® 680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S5
Modification Site Ser5
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5430
Swiss Prot P24928
Subcellular location Nucleus
Synonyms POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS.
Background During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200