Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Monoclonal Antibody

$Western blot analysis of HeLa Cell lysate treated with lambda phosphatase. Using Phospho-PAK1\/2\/3 (Ser144\/Ser141\/Ser154) (bsm-62927R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HeLa Cell lysate treated with lambda phosphatase. Using Phospho-PAK1\/2\/3 (Ser144\/Ser141\/Ser154) (bsm-62927R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62927r-100ul
Product Name	Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC), IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PAK1 around the phosphorylation site of S144
Modification Site	Ser144/Ser141/Ser154
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5058
Swiss Prot	Q13153,Q13177,O75914
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ADRB2; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1 (phospho S144); PAK2 (phospho S141); PAK3 (phospho S139).
Background	Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IP	1-2ug
IF