Phospho-Nucleolin (Thr84) Monoclonal Antibody

Overview
Catalog #	bsm-62916r-100ul
Product Name	Phospho-Nucleolin (Thr84) Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T84
Modification Site	Thr84
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4691
Swiss Prot	P19338
Subcellular location	Cytoplasm, Nucleus
Synonyms	NCL; Nucl; Nucleolin; Protein C23.
Background	Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Application Dilution
WB	:1:1000-1:2000
IHC-P	IHC-P:1:100-1:200
IHC-F	IHC-F:1:100-1:200
IF	IF:1:100-1:200