MAPKAP Kinase 2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with MAPKAP Kinase 2 monoclonal antibody, unconjugated (bsm-62903R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MAPKAP Kinase 2 monoclonal antibody, unconjugated (bsm-62903R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62903r-100ul
Product Name	MAPKAP Kinase 2 Recombinant Antibody
Applications	WB, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MK2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9261
Swiss Prot	P49137
Subcellular location	Cytoplasm, Nucleus
Synonyms	MAPKAP K2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK2; Mitogen activated protein kinase activated protein kinase 2; MK 2.
Background	Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue.
Application Dilution
WB	1:300-5000
IP	1-2ug